Transforming Growth Factor β1 (TGF-β1) is secreted in a latent, biologically inactive form, non-covalently linked with a Latency Associated Protein (LAP). The monoclonal antibodies MT593 and MT517-biotin bind to the LAP entity of human latent TGF-β1. This enables direct quantification of latent TGF-β1 in ELISA with no need of sample pre-treatment. The ELISA does not detect human latent TGF-β2 or -β3 or bovine latent TGF-β1.
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
ELISA kit for quantitative determination of native and recombinant human latent TGF-β1 in e.g. cell culture supernatants. The kit contains capture mAb, biotinylated detection mAb, streptavidin-enzyme conjugate ALP and standard. The determination of latent TGF-β1 in plasma samples by this kit requires the use of ELISA diluent (code: 3652-D2).