Mouse anti-Claudin-5; Unconjugated Monoclonal antibody specific to Human, Mouse, Rat Claudin-5 . This antibody is validated for use in Western Blot, Immunohistochemistry, Immunoassay (ELISA), Immunocytochemistry/ Immunofluorescence. Anti-Claudin-5 recognizes the expressed product of the CLDN5 gene, also known as Cldn5.
USAGE
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.1-1.0 μg/mL
Western Blotting: 1-3 μg/mL
Immunohistochemistry*: 5-10 μg/mL
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