Tumor necrosis factor-α (TNF-α) is produced by many different cell types e.g. monocytes, macrophages, T cells and B cells. Among the many effects of TNF-α, are protection against bacterial infection, cell growth modulation, immune system regulation and involvement in septic shock.
All kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
ELISA kit for quantitative determination of native and recombinant human TNF-α in biological fluids such as cell culture supernatants. The kit contains capture mAbs, biotinylated detection mAb, streptavidin-enzyme conjugate ALP and a cytokine standard. For determination of TNF-α in human serum/plasma samples we recommend the use of our ELISA diluent (3652-D2).