Perforin is produced by cytotoxic T cells and NK cells as an effector molecule in the cell-mediated destruction of target cells. Perforin will facilitate delivery of granzymes which induces apoptosis of the target cell.
All kits include a Perforin standard derived from human YT cells. No international standard exists for calibration.
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
ELISA kit for quantitative determination of human Perforin in biological fluids such as serum, plasma and cell culture supernatants. The kit contains capture mAb, biotinylated detection mAb, streptavidin-enzyme conjugate ALP and a Perforin standard derived from human YT cells. The determination of Perforin in human serum/plasma samples by this kit requires the use of ELISA diluent (code: 3652-D2) for dilution of samples, standard and detection antibody.