Interleukin-12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes TH1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells.
Please check our different ELISA kits for the detection of human IL-12 p70 specific below. All kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
ELISA kit for quantitative determination of native and recombinant human IL-12 (p70) heterodimer (p40+p35) in biological fluids such as serum, plasma and cell culture supernatants. The kit contains capture mAb, biotinylated detection mAb, streptavidin-enzyme conjugate HRP and a recombinant IL-12 standard (p70 heterodimer). The determination of IL-12 (p70) heterodimer (p40+p35) in human serum/plasma samples by this kit requires the use of ELISA diluent (code: 3652-D2) for dilution of samples, standard and detection antibody.