Interleukin-12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes TH1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells.
Reacts with Human, cross reacts with certain non-human primates. Neutralizing antibodies have only been tested with human cells.
Mouse monoclonal antibodies specific for the p40 subunit of human Interleukin-12 and Interleukin-23. The reagent consists of a combination of two monoclonal antibodies. The antibodies recognize p40 monomer, p40 homodimer, IL-12 p70 heterodimer (p40+p35) and IL-23 heterodimer (p40+p19).
Suitable as capture antibodies in ELISA and ELISpot for detection of:
- IL-12 and IL-23 in combination with mAb MT618 biotinylated ( code 3450-6)
- IL-12 p70 heterodimer (p40+p35) in combination with mAb IL12-III biotinylated (code 3450-8)
- IL-23 heterodimer (p40+p19) in combination with mAb MT155 biotinylated (code 3457-6)