Interleukin-12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells.
Interleukin-23 (IL-23) is a disulfide-linked heterodimer which consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells and IL-23 is responsible for Th17 expansion and stabilization.
Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.
All ELISA kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
ELISA kit for quantitative determination of native and recombinant human IL-12 and IL-23 in biological fluids such as serum, plasma and cell culture supernatants. The kit contains capture mAb, biotinylated detection mAb, streptavidin-enzyme conjugate HRP and a recombinant IL-12 standard (p70 heterodimer). The determination of IL-12 and IL-23 in human serum/plasma samples by this kit requires the use of ELISA diluent (code: 3652-D2) for dilution of samples, standard and detection antibody.
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