Interferon-alpha (IFN-α) is produced by multiple cell types in response to viral infection and constitutes an important part of the innate immune response. There are multiple human IFN-α genes of which at least 13 are transcribed. This results in 12 different functional IFN-α subtype proteins since IFN-α 1 and IFN-α 13 are identical. The IFN-α subtypes display high homology with each other but differ to various extents with regard to the type of producer cells and their anti-viral capacity. The subtype specificity of antibodies to IFN-α differs and needs to be defined for each antibody or for each capture/detection antibody combination. The pan specific system is based on multiple antibodies to detect 11 out of 12 IFN-α subtypes (1, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17).
All kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
Mabtech’s enzyme-linked immunosorbent assay, ELISA PRO, is a complete kit for the quantification of all human IFN-α subtypes except subtype 21 in biological fluids such as serum, plasma and cell culture supernatants. The assay utilizes ELISA strip plates precoated with capture monoclonal antibodies (mAbs), biotinylated mAbs for detection, streptavidin-horseradish peroxidase (SA-HRP), TMB substrate, cytokine standard, buffers, diluents, stop solution and adhesive plate covers.
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