Interferon-α is produced by multiple cell types in response to viral infection and constitutes an important part of the innate immune response to infection. There are multiple human IFN-α genes of which at least 13 are transcribed. This results in 12 different functional IFN-α subtype proteins since IFN-α 1 and IFN-α 13 are identical. The IFN-α subtypes display high homology with each other but differ to various extents with regard to the type of producer cells and their anti-viral capacity. The subtype specificity of antibodies to IFN-α differs and needs to be defined for each antibody or for each capture/detection antibody combination. The below mentioned kits are suitable for the detection of human IFN-α subtype 2.
Please check our different ELISA kits for the detection of human IFN-alpha subtype 2 below. All kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
ELISA kit for quantitative determination of human IFN-α subtypes 2a, 2b and 2c in biological fluids such as serum, plasma and cell culture supernatants. The kit contains capture mAb MT1, biotinylated detection mAb MT2, streptavidin-enzyme conjugate ALP and a cytokine standard. The determination of IFN-α in human serum/plasma samples by this kit requires the use of ELISA diluent (code: 3652-D2) for dilution of samples, standard and detection antibody.
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