Interferon-γ (IFN-γ) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Cross-reactivity has been tested and confirmed with certain non-human primate species.
ELISpot PRO kit for the detection of human IFN-γ. The kit contains precoated transparent plates, ALP-labelled detection antibody (one-step detection), BCIP/NBT substrate and anti-CD3 mAb as positive control. Combines excellent performance with rapid procedure and high reproducibility. Assay time is approximately 3,5 hours excluding cell incubation time.
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