IFN-γ (Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ (Interferon-gamma) by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ (Interferon-gamma) is typically associated with effective host defense against intracellular pathogens.
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
All kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
ELISA kit for quantitative determination of native and recombinant human IFN-γ in biological fluids such as serum, plasma and cell culture supernatants. The kit contains capture mAb, biotinylated detection mAb, streptavidin-enzyme conjugate ALP and a cytokine standard. The determination of IFN-γ in human serum/plasma samples by this kit requires the use of ELISA diluent (code: 3652-D2) for dilution of samples, standard and detection antibody.