Interleukin-4 (IL-4) is produced by activated T cells, especially differentiated CD4+ TH2-type cells, and stimulated mast cells and basophils. The most important effects of IL-4 are the regulation of helper T cell differentiation to the TH2 type and the regulation of IgE and IgG4 production by B cells.
Cross-reactivity has been tested and confirmed with certain non-human primate species.
ELISpot PRO kit for the detection of human or monkey IL-4. The kit contains precoated white plates, ALP-labelled detection antibody (one-step detection), BCIP/NBT substrate and anti-CD3 mAb as positive control. Combines excellent performance with rapid procedure and high reproducibility. Assay time is approximately 3,5 hours excluding cell incubation time.
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