Interleukin-4 (IL-4) is produced by activated T cells, especially differentiated CD4+ TH2-type cells, and stimulated mast cells and basophils. The most important effects of IL-4 are the regulation of helper T cell differentiation to the TH2 type and the regulation of IgE and IgG4 production by B cells.
All kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
Mabtech’s enzyme-linked immunosorbent assay, ELISA PRO, is a complete kit for the quantification of human IL-4 in biological fluids such as serum, plasma and cell culture supernatants. The assay utilizes ELISA strip plates precoated with a capture monoclonal antibody (mAb), a biotinylated mAb for detection, streptavidin-horseradish peroxidase (SA-HRP), TMB substrate, cytokine standard, buffers, diluents, stop solution and adhesive plate covers.
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