Interleukin-4 (IL-4) is produced by activated T cells, especially differentiated CD4+ TH2-type cells, and stimulated mast cells and basophils. The most important effects of IL-4 are the regulation of helper T cell differentiation to the TH2 type and the regulation of IgE and IgG4 production by B cells.
All kits include a recombinant cytokine standard calibrated against an international standard (NIBSC).
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
ELISA kit for quantitative determination of native and recombinant human IL-4 in biological fluids such as serum, plasma and cell culture supernatants. The kit contains capture mAb, biotinylated detection mAb, streptavidin-enzyme conjugate HRP and a cytokine standard. The determination of IL-4 in human serum/plasma samples by this kit requires the use of ELISA diluent (code: 3652-D2) for dilution of samples, standard and detection antibody.
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