Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Conjugate
Alexa Fluor® 488
Form
Liquid
Concentration
0.5 mg/ml
Purification
purified
Storage buffer
PBS, pH 7.4, with 1% BSA, 50% glycerol
Preservative
0.1% sodium azide
Storage Conditions
4° C, store in dark
Tested Applications
Dilution *
ELISA (ELISA)
0.1-1.0 ug/ml
Immunocytochemistry (ICC)
Assay Dependent
Immunofluorescence (IF)
5-10 ug/ml
Western Blot (WB)
1-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
