Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ELISA (ELISA)
0.1-1.0 ug/ml
Flow Cytometry (Flow)
3-5 µg/million cells
Immunofluorescence (IF)
Assay Dependent
Immunohistochemistry (Frozen) (IHC (F))
5-10 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:50
Immunoprecipitation (IP)
5 ug
Western Blot (WB)
0.5-2 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor , which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.