A His-tagged Plk fusion protein (C-term Plk1) containing amino acids 402-603 of the human Plk1 protein(
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ELISA (ELISA)
0.1-1.0 ug/ml
Flow Cytometry (Flow)
3-5 µg/million cells
Immunocytochemistry (ICC)
1-5µg/mL
Immunofluorescence (IF)
1-5µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Immunoprecipitation (IP)
5 ug
Western Blot (WB)
1-2 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
33-1700 contains a cocktail of monoclonal mouse PLK1 containing clone PL6, isotype IgG1, kappa and clone PY20, isotype IgG2b.
Background/Target Information
Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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