Gibco® Opti-MEM®培养基使用碳酸氢钠缓冲体系(2.4 g / L),要求在5-10% CO2环境下培养细胞。
原厂资料:
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction ofFetal Bovine Serumsupplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM®modificationsfor a range of cell culture applications.
This Opti-MEM® is modified as follows:
With
• L-glutamine
• Phenol Red
The complete formulation is confidential. For more information, please contactTechnical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Product Intended Use Forin vitrodiagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® Opti-MEM® product made in our Scotland facility (31985-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g / L) and therefore requires a 5-10% CO2environment to maintain physiological pH.