RPMI 1640 1X50L

产品编号：31800105 品牌：invitrogen BP 原厂货号：31800105
产品分类：细胞,细胞培养与转染试剂 > 基础培养基 > RPMI 1640
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞培养基 > RPMI 1640

包装：

运保温度:

2-8° C

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描述：

RPMI 1640 最初用于培养人白血病细胞。RPMI 1640已被证实适合培养多种哺乳动物细胞如HeLa, Jurkat, MCF-7, PC12, PBMC, 星形胶质细胞和肿瘤细胞等。

RPMI 配方改良如下：

With	Without
• L-glutamine	• HEPES
• Phenol Red	Sodium Bicarbonate

可查看完整的配方。31800 - RPMI 1640, powder

区别于其他培养基Gibco® RPMI 1640 中含有还原剂谷胱甘肽和高浓度的维生素。与 EMEM、DMEM相比PMI 1640 还含有生物素,维生素B12和PABA。另外，维生素肌醇、胆碱含量非常高。

RPMI 1640不含蛋白质、脂质和生长因子。因此RPMI 1640需要补充添加剂，一般是10%胎牛血清。RPMI 1640 使用碳酸氢钠缓冲体系(2.0 g⁄L),因此需要5 - 10%的二氧化碳培养环境保持生理pH值。

原厂资料：

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With	Without
• L-glutamine	• HEPES
• Phenol Red	• Sodium Bicarbonate

The complete formulation is available.

Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂ and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g / L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).