305005 reacts with the heavy and light chains of rat IgG, and the light chains of rat IgA and IgM. Cross reactivity with mouse immunoglobulins has been minimised by cross adsorption with pooled mouse sera and plasmacytoma/hybridoma proteins.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/4000 - 1/8000
Immunoblotting
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antiserum to rat IgG was raised by repeated immunisation of goats with highly purified antigen.
Purified IgG was prepared by affinity chromatography.
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