302009 reacts with the heavy chain of rat IgM. The antibody may cross react with IgM from other species.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat - 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antiserum to rat IgM was raised by repeated immunisation of goats with highly purified antigen. The preparation was purified by affinity chromatography on rat IgM covalently linked to agarose.