2470-5386 detects cytomegalovirus early nuclear antigen. Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is also commonly known as human herpesvirus 5 (HHV-5). Early HCMV proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection, prior to viral DNA replication.
2470-5386 is also reactive with MRC-5 cells infected with CMV, but not uninfected cells.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Suitable for methacam fixed, paraffin wax embedded sections as well as acetone fixed CMV infected cells or frozen sections.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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