Clone Lu-5 recognizes an intracytoplasmic, formaldehyde-resistant epitope on the surface of cytokeratin filaments.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Chicken
Yes
Horse
Yes
Rat
Yes
Xenopus
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
1/500 - 1/1000
Immunohistology - Paraffin(1)
1/200 -
(1)
This product requires protein digestion pre-treatment of paraffin sections. Proteinase K is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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