C3 consists of two polypeptide chains, an alpha chain and a beta chain. It is the pivotal protein of the complement system, being central in the classical pathway and the alternative pathway. C3 is cleaved by the classical pathway convertase C4b2a and the alternative pathway convertase C3bBb, into C3b and C3a. C3c is formed as a result of inactivation of C3b by Factor 1 and other cofactors.
2222-6604F recognises the C3c component of human complement, formed as a result of the inactivation of C3b.The reagent may be used for the detection of C3 deposits in tissues following complement activation.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/500 - 1/50K
Immunofluorescence
1/10 - 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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