2115-5004 is specific for clathrin and stains coated vesicles from various organs and tissues tested e.g. brain, mammary gland, ovaries. It also reacts with the cell lines, HeLa, SV-40 and RVF-SMC.
Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. It is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows clathrin to form its unique polyhedral network.
In nervous tissue, 2115-5004 cross-reacts with another coated vesicle protein of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species, this antibody may react with a cytokeratin component.
Species Cross-Reactivity
Target Species
Cross Reactivity
Amphibia
Yes
Pig
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
1/10 - 1/50
Western Blotting
1/50 - 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
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