HAT添加剂(50X),次黄嘌呤钠、氨喋呤和胸腺嘧啶的液体混合物，用于杂交瘤培养

• 产品编号：21060017      品牌：invitrogen       原厂货号：21060017
• 产品分类：细胞,细胞培养与转染试剂 > 添加剂 > 用于杂交瘤细胞的添加剂
• 应用分类：暂定分类 > 通用培养基添加剂 > HAT Supplement and HT Supplement > HAT Supplement

描述：

HAT 添加剂 (HAT Supplement) (50X) 是次黄嘌呤钠、氨喋呤和胸腺嘧啶的液体混合物。 稀释后的 HAT 适合用作融合后的选择培养基，以去除未融合或自融合的 HGPRT--骨髓瘤细胞。 次黄嘌呤和胸腺嘧啶提供预先形成的嘌呤和嘧啶，以便杂交瘤通过补救途径合成 DNA，补救途径会利用脾脏融合细胞提供的 HGPRT。 氨喋呤是叶酸拮抗剂，可以抑制核苷的从头生物合成途径。

浓度为 50X： 5mM 次黄嘌呤钠，20uM 氨喋呤，0.8mM 胸腺嘧啶

原厂资料：

Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Dual site cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® HAT Supplement at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

注意事项：