Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM ⁄ F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM ⁄ F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM ⁄ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
Gibco® DMEM ⁄ F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids and vitamins with F-12’s wide variety of
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® DMEM ⁄ F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.
DMEM ⁄ F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM ⁄ F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM ⁄ F-12 uses a sodium bicarbonate buffer system (2.438 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
原厂资料：
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
For supply chain continuity, Life Technologies manufactures DMEM/F-12 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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