2090-1439 is specific for the beta subunit of human chorionic gonadotropin and also reacts with hLH beta subunit. It does not recognise hGH or hCG alpha subunit. Minimal reactivity has been observed with hTSH and hFSH.
Human chorionic gonadotrophin is a 30kD hormone produced by placental syncytiotrophoblasts. hCG acts to maintain the corpus luteum until the developing placenta is able to produce the required levels of oestrogen and progesterone.
hCG shares a common alpha chain with the pituitary hormones FSH, TSH and LH, but has a unique beta chain which is recognised by this antibody.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
1/200 - 1/500
Immunohistology - Paraffin
Western Blotting
1/300 - 1/400
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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