BIOPRIME CGH LABELING SYSTEM 30 REACTIONS

• 产品编号：18095011      品牌：invitrogen       原厂货号：18095011
• 产品分类：DNA和RNA纯化 > 核酸标记 > DNA标记
• 应用分类：核酸分离纯化与分析 > 核酸标记 > DNA 标记

描述：

The BioPrime® Plus Array CGH Genomic Labeling Systems are a high-performance labeling kits that enable reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH). Available in both indirect and direct labeling formats, the BioPrime® Plus Array CGH Genomic Labeling Systems provide a flexible solution to your genomic labeling needs. Using the
BioPrime® Plus Array CGH Genomic Labeling Systems, you can expect:
––High yields of fluorescently labeled genomic DNA
–signal-to-noise ratios
–detection of gene copy number variations (Figure 1)


These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5´ - 3´ and 3´ - 5´ exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor® AHA fluorescent nucleotides. In the BioPrime® Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor® AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor® NHS ester (indirect labeling systems) prior to hybridization (Figure 2).

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