The SuperScript® III CellsDirect cDNA Synthesis Kit provides high yields of first-strand cDNA directly from cells, minimizing sample loss from RNA isolation procedures and ensuring accurate representation of all transcripts. The system uses SuperScript® III RT to synthesize cDNA with increased specificity, produce higher yields of cDNA, and synthesize full-length products up to 4.5 kb.

The SuperScript® III CellsDirect cDNA Synthesis Kit offers:

• High yield cDNA synthesis from 1 to 10,000 cells (Figure 1)

• Time savings-go from cells to cDNA in less than 2 hours

• A single tube format without a separate RNA purification step-eliminates sample loss and enables detection of rare transcripts

• Compatibility with a wide variety of applications, including PCR, real-time PCR, and siRNA