Taq DNA Polymerase is a thermostable enzyme that can withstand prolonged incubation at temperatures up to 95“C without significant loss of activity. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´a3´ DNA polymerase activity and a 5´a3´ exonuclease activity (Figure 1). With Taq DNA Polymerase you'll get:
Your choice of recombinant or native enzyme
Amplification of PCR products up to 5 kb in size
An enzyme that is licensed and qualified for PCR
Taq PCRx DNA Polymerase is Taq DNA Polymerase supplied with the PCRx Enhancer System for optimizing PCR of problematic and/or GC-rich templates. 10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Taq DNA polymerase.
Applications: Amplification of DNA from 查看更多complex genomic, viral, and plasmid templates; RT-PCR (1); sequencing ssDNA (2); and cycle sequencing (3,4).
Source: Native: Purified from Thermus aquaticus YT1. Recombinant: Purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Unit Definition: One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min. at 74°C.
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For Research Use Only. Not for use in diagnostic procedures.