LARGE FRAGMENT 500 UN

产品编号：18012039 品牌：invitrogen 原厂货号：18012039
产品分类：克隆 > 聚合酶 > DNA聚合酶
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-20°C

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描述：

DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase that Polymerase I, but it contains the 3´ 5´ exodeoxyribonuclease activity.

Applications: Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.

Source: Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing: SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a
template•primer.

原厂资料：

DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase that Polymerase I, but it contains the 3´ 5´ exodeoxyribonuclease activity.

Applications: Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.

Source: Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing: SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a
template•primer.

注意事项：

For Research Use Only. Not for use in diagnostic procedures.