B-27® Supplement is an optimized serum-free supplement used to support the low or high density growth and short or long-term viability of hippocampal and other CNS neurons. B-27® Supplement is provided as a 50X liquid and is intended to be used with Neurobasal® Medium or Neurobasal®-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.
B-27® Supplement is suitable for use in most neuronal cell applications. For specialized applications and depending on your experimental needs, we provide alternative formulations of B-27® Supplement.
Quality control testing
B-27® Supplement is tested in a growth assay utilizing primary rat (Sprague Dawley) embryonic hippocampal neurons, 18 day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27® Supplement is also tested for endotoxin at a 1X concentration.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.