Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
10 U/ µL
Contents
SpeI 10X Buffer M (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
37°C
Reaction Mixture
SpeI 1 µL 10X Buffer M 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme is inactivated by heating at 80°C for 20 minutes.
Star Activity
Unrelated site may be cut at low ionic strength.
Effect of DNA Methylation
Enzyme activity is unaffected by DNA methylation.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer M
100 mM Tris-HCl, pH 7.5 100 mM MgCl210 mM Dithiothreitol 500 mM NaCl
Source
Sphaerotilus natans
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
80*
100
80
100
80*
*Weak star activity is detected.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
原厂资料：
5´-A↓CTAGT-3´ 3´-TGATC↑A-5´
Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
10 U/ µL
Contents
SpeI 10X Buffer M (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
37°C
Reaction Mixture
SpeI 1 µL 10X Buffer M 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme is inactivated by heating at 80°C for 20 minutes.
Star Activity
Unrelated site may be cut at low ionic strength.
Effect of DNA Methylation
Enzyme activity is unaffected by DNA methylation.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer M
100 mM Tris-HCl, pH 7.5 100 mM MgCl210 mM Dithiothreitol 500 mM NaCl
Source
Sphaerotilus natans
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
80*
100
80
100
80*
*Weak star activity is detected.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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