Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
10 U/ µL
Contents
ScaI 10X Buffer H (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
37°C
Reaction Mixture
ScaI 1 µL 10X Buffer H 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme is inactivated by heating at 65°C for 20 minutes.
Star Activity
Unrelated site may be cut in the presence of high concentrations of glycerol.
Effect of DNA Methylation
Enzyme activity is not affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer H
500 mM Tris-HCl 100 mM MgCl210 mM Dithiothreitol 1,000 mM NaCl
Source
Streptomyces achromogenes
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
<20*
<20*
100
60*
<20*
*weak star activity detected
原厂资料：
5´-AGT↓ACT-3´ 3´-TCA TGT↑A-5´
Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
10 U/ µL
Contents
ScaI 10X Buffer H (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
37°C
Reaction Mixture
ScaI 1 µL 10X Buffer H 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme is inactivated by heating at 65°C for 20 minutes.
Star Activity
Unrelated site may be cut in the presence of high concentrations of glycerol.
Effect of DNA Methylation
Enzyme activity is not affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer H
500 mM Tris-HCl 100 mM MgCl210 mM Dithiothreitol 1,000 mM NaCl
Source
Streptomyces achromogenes
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
<20*
<20*
100
60*
<20*
*weak star activity detected
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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