Lipofectamine® LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest possible transfection efficiencies and viabilities. Lipofectamine® LTX Reagent is a new, advanced solution for gene expression in both common and challenging cell types. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, challenging, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support.
Lipofectamine® LTX Reagent offers:
• Superior delivery – transfect a broad range of cell types, including primary challenging and sensitive cells, with high efficiency • Superior performance – combine high transfection efficiency with maximal (>90%) cell viability • Optimized protocols – rapid, simple protocols provided for most cell lines • A highly effective reagent to generate stable cell lines
Transfect Primary Cells Efficiently Primary cells are often difficult to transfect. Lipofectamine® LTX Reagent, when combined with PLUS™ Reagent, provides the highest level of transfection in primary cells of any lipid- or polymer-based reagent. Many primary cell types have been successfully transfected with Lipofectamine® LTX including primary neural progenitor cells.
Superior Delivery into Challenging Cells When it comes to achieving high levels of protein expression in RAW267.3 macrophages, Lipofectamine® LTX Reagent outperforms competing transfection reagents (see Figure 4). Achieving high-level transgene expression in cells that are traditionally refractory to transfection allows you to obtain results that were previously unattainable.
Broad Compatibility with Cell Lines In addition to being excellent for transfecting primary and challenging cells, Lipofectamine® LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents. Use our Transfection Selection Tool to help you select the best transfection conditions for the cells with which you work.
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For Research Use Only. Not for use in diagnostic procedures.