Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
15 U/ µL
Contents
SacII 10X K (1 µL) 0.1%BSA 10X loading dye (1 µL)
Reaction Temperature
37°C
Reaction Mixture
SacII 1 µL 10X T Buffer 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme is inactivated by heating at 65°C for 20 minutes.
Star Activity
Effect of DNA Methylation
Enzyme activity is affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Reaction Buffer M
10X Buffer T (BSA-free) 330 mM Tris-AC, pH 7.9 100 mM Mg-Ac 5 mM Dithiothreitol 660 mM K-Ac
Source
Streptomyces achromogenes
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
40
20
<20
<20
100
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
原厂资料：
5´-CCGC↓GG-3´ 3´-GG↑CGCC-5´
Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
15 U/ µL
Contents
SacII 10X K (1 µL) 0.1%BSA 10X loading dye (1 µL)
Reaction Temperature
37°C
Reaction Mixture
SacII 1 µL 10X T Buffer 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme is inactivated by heating at 65°C for 20 minutes.
Star Activity
Effect of DNA Methylation
Enzyme activity is affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Reaction Buffer M
10X Buffer T (BSA-free) 330 mM Tris-AC, pH 7.9 100 mM Mg-Ac 5 mM Dithiothreitol 660 mM K-Ac
Source
Streptomyces achromogenes
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
40
20
<20
<20
100
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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