Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
10 U/ µL
Contents
SmaI 10X Buffer T (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
30°C
Reaction Mixture
SmaI 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme inactivated by heating at 65°C for 20 minutes.
Star Activity
Unrelated site may be cut in the presence of high concentration of glycerol.
Effect of DNA Methylation
Enzyme is affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 30°C.
10X Buffer T
330 mM Tris-AC, pH 7.9 100 mM Mg-Ac 5 mM Dithiothreitol 660 mM K-Ac
Source
Serratia marcescensSb
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
<20
<20
<20
<20
100
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
原厂资料：
5´-CCC↓GGG-3´ 3´-GGG↑CCC-5´
Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
10 U/ µL
Contents
SmaI 10X Buffer T (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
30°C
Reaction Mixture
SmaI 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation
Enzyme inactivated by heating at 65°C for 20 minutes.
Star Activity
Unrelated site may be cut in the presence of high concentration of glycerol.
Effect of DNA Methylation
Enzyme is affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 30°C.
10X Buffer T
330 mM Tris-AC, pH 7.9 100 mM Mg-Ac 5 mM Dithiothreitol 660 mM K-Ac
Source
Serratia marcescensSb
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
<20
<20
<20
<20
100
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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