Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
15 U/ µL
Contents
EcoR I 10X Buffer H (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
37°C
Reaction Mixture
EcoR I 1 µL 10X Buffer H 2 µL Substrate DNA < 1 µg Sterile water to 20 µL
Heat inactivation
Enzyme inactivated by heating at 65°C for 20 minutes.
Star Activity
Unrelated site may be cut in the presence of high concentrations of glycerol or Mn2+, and at low ionic strength. Enzyme activity reduces to 70-80% by the addition of spermine (0.2mM), but star activity reduces to 30-50%.
Effect of DNA Methylation
The sequence GAATTCG is affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer H
500 mM Tris-HCl, pH 7.5 100 mM MgCl210 mM Dithiothreitol 1,000 mM NaCl
Source
Escherichia coliRY 13
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
20*
100*
100
120*
80*
* weak star activity is detected For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
原厂资料：
5´-G↓AATTC-3´ 3´-CTTAA↑G-5´
Important:Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Concentration
15 U/ µL
Contents
EcoR I 10X Buffer H (1mL) 10X Loading Buffer (1mL)
Reaction Temperature
37°C
Reaction Mixture
EcoR I 1 µL 10X Buffer H 2 µL Substrate DNA < 1 µg Sterile water to 20 µL
Heat inactivation
Enzyme inactivated by heating at 65°C for 20 minutes.
Star Activity
Unrelated site may be cut in the presence of high concentrations of glycerol or Mn2+, and at low ionic strength. Enzyme activity reduces to 70-80% by the addition of spermine (0.2mM), but star activity reduces to 30-50%.
Effect of DNA Methylation
The sequence GAATTCG is affected by CG methylase.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer H
500 mM Tris-HCl, pH 7.5 100 mM MgCl210 mM Dithiothreitol 1,000 mM NaCl
Source
Escherichia coliRY 13
Relative Activity in Universal Reaction Buffers
Buffer
L
M
H
K
T(+BSA)
Relative Activity (%)
20*
100*
100
120*
80*
* weak star activity is detected For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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