Description: RMUL.2 is a monoclonal antibody that reacts with the predicted extracellular domain of mouse Langerin (CD207). Mouse Langerin(CD207) is expressed in a distinct subset of Dendritic cells called Langerhans cells (LC). Mouse Langerin is a 48kDa C-type lectin putative transmembrane protein that likely plays a role in antigen recognition and uptake. Langerhans cells are located in the epidermis and upon activation, reduce Langerin expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse Langerin in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of Langerin cDNA into fibroblast cell lines.
Western Blotting with RMUL.2 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse Langerin cDNA. Cross reactivity of this antibody with other proteins has not been determined. Preliminary data from mouse Langerin cDNA transfected cells suggests that RMUL.2 can be used for intracellular flow cytometric analysis. We have observed in our transfectants the majority of protein is expressed intracellularly with a small percentage on the cell surface. In addition, RMUL.2 has also been tested by immunohistology on mouse ear epidermal sheets and shows specific staining when compared to a polyclonal antibody obtained from a different supplier.
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