The major use of the glass fiber filters is as a depth prefilter, placed directly on top of a membrane filter, whereby the prefilter diameter specified for the holder must be chosen. Larger diameters would intrude under the sealing ring of the holder and cause leakage. The standard type 13400 contains an acrlic binder. It has a high particle loading capacity, but for very polluted liquids the thicker type 13430 can be more effective. 13440 is a finer, binder-free type and is recommended for the prefiltration of relative clean solutions such as tissue culture media. Serial filtration may be necessary for difficult-to-filter liquids such as serum.
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