USAGE
This antibody is suitable for immunohistochemical staining of alcohol or paraformaldehyde fixed paraffin-embedded or frozen tissue sections. Heat-induced epitope retrieval with citrate buffer, pH 6.0, is required for specific staining of formalin-fixed, paraffin-embedded tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C. This antibody may also be used in ELISA and Western blotting.
ELISA: 0.1-1 μg/mL
Western Blotting*6-15, 18-19: 1-3 μg/mL
Immunofluorescence16-17: 2-5 μg/mL