Manufactured by Sino Biological (China); Distributed by Life Technologies.
Green Fluorescent Protein (GFP) recombinant Aequorea victoria protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein structure analysis and in cell biology research applications.
This recombinant protein is expressed from a DNA sequence encoding the Aequorea victoriaGFP (AAB65663) (Ser 2-Lys 238) fused to a polyhistidine tag at the N-terminus.
N-terminal Sequence Analysis: Glu 99.
Activity: 1. Mesured by emission analysis. 2. Measure excitation and emission spectra of GFP at wavelengths of 395 nm and 510 nm respectively.
Formulation: Lyophilized from sterile PBS, pH 7.5.
Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Expiration Date: Expires one year from date of receipt when stored as instructed.
Background The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of β-sheet with an α-helix inside and short helical segments on the ends of the cylinder. Its ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has long been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
