Specifically recognizes a phosphate-independent epitope on NF-L.
Conjugate
Unconjugated
Form
Liquid
Purification
purified
Storage buffer
PBS
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ELISA (ELISA)
0.1-0.5 ug/ml
Immunocytochemistry (ICC)
1:20
Immunofluorescence (IF)
1:20
Immunohistochemistry (IHC)
1:20 - 1:100
Immunoprecipitation (IP)
2-5 ug
Western Blot (WB)
0.5-2 ug/mL
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
13-0400 detects the neurofilament, light chain in human, rat, and mouse samples.
Background/Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa.
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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