The Naive CD4+ T Cell Isolation Kit has been developed for the isolation of untouched naive CD4+ T helper cells from suspensions of mouse spleen cells or lymph node cells. Non-naive CD4+ T cells, i.e., cytotoxic T cells, regulatory T cells, activated T cells, B cells, NK cells, macrophages, granulocytes, endothelial cells, and erythroid cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Memory T cells are directly magnetically labeled with CD44 MicroBeads. Isolation of highly pure naive CD4+ T cells is achieved by depletion of magnetically labeled non-target cells.
原厂资料:
The Naive CD4+ T Cell Isolation Kit has been developed for the isolation of untouched naive CD4+ T helper cells from suspensions of mouse spleen cells or lymph node cells. Non-naive CD4+ T cells, i.e., cytotoxic T cells, regulatory T cells, activated T cells, B cells, NK cells, macrophages, granulocytes, endothelial cells, and erythroid cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Memory T cells are directly magnetically labeled with CD44 MicroBeads. Isolation of highly pure naive CD4+ T cells is achieved by depletion of magnetically labeled non-target cells.