The CD4+CD25+Regulatory T Cell Isolation Kit, mouse was developed for the isolation of CD4+CD25+regulatory T cells (Treg) from single-cell suspensions of mouse spleen and lymph nodes. The isolation is performed in a fast two-step procedure.
原厂资料:
The CD4+CD25+ Regulatory T Cell Isolation Kit has been developed for the isolation of mouse CD4+CD25+ regulatory T cells from single-cell suspensions of spleen or lymph nodes without centrifugational washing steps during the separation.
CD4+CD25+ immunoregulatory T cells have been shown to actively suppress immune responses against autologous and foreign antigens in vivo and in vitro. CD25, the IL-2Rα chain, is also expressed on activated CD8+ T cells, dendritic cells (DCs), and B cells. The kit contains a cocktail of lineage specific biotin-conjugated antibodies against CD8, CD11b, CD45R, CD49b, Ter-119, and AntiBiotin MicroBeads for depletion of non-CD4+ T cells, as well as CD25-PE and Anti-PE MicroBeads for subsequent positive selection of CD4+CD25+ regulatory T cells.