干粉培养基DMEM，盒装，无HEPES、碳酸氢钠

产品编号：12800017 品牌：invitrogen 原厂货号：12800017
产品分类：细胞,细胞培养与转染试剂 > 基础培养基 > DMEM
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞培养基 > DMEM

包装：

10X1L

运保温度:

2-8° C

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描述：

DMEM是广泛使用的哺乳动物细胞培养基。成纤维细胞、神经细胞、胶质细胞、人脐静脉内皮细胞、平滑肌细胞都可使用DMEM进行培养。

DMEM 配方改良如下：

With	Without
• High Glucose	• HEPES
• L-glutamine	• Sodium Bicarbonate
• Phenol Red
• Sodium Pyruvate

可查看完整的配方。12800 - DMEM, powder, high glucose, pyruvate

DMEM 氨基酸和维生素浓度是传统 Eagle's Minimal 查看更多Essential Medium（EMEM）的四倍。 DMEM 最初配制含较低浓度的葡萄糖(1 g⁄L)和丙酮酸钠, 目前使用的DMEM多为高糖、有/无丙酮酸钠。

DMEM不含蛋白质、脂类、生长因子等。因此使用时需添加补充添加剂，一般为10%FBS。DMEM使用碳酸氢钠缓冲体系(3.7 g / L)，因此在 5-10% CO₂ 环境下培养细胞。

原厂资料：

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• HEPES
• L-glutamine	• Sodium Bicarbonate
• Phenol Red
• Sodium Pyruvate

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).