BGJb medium was developed for the growth of fetal rat long bones in a chemically defined medium. BGJb is a medium modified by Sylvia Fitton-Jackson from the original BGJ medium developed by Biggers, Gwatkin, and Judah. BGJb medium creates favorable conditions for growth and calcification of fetal bones.
This BGJb medium is manufactured as follows:
With
Without
• L-glutamine
• HEPES
• Phenol Red
The complete formulation is available.
Gibco® BGJb medium contains higher levels of amino acids and glucose, and an increased buffering capacity to improve the conditions for growth and calcification of fetal bone.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
cGMP Manufacturing
Gibco® BGJb medium is manufactured at a cGMP 查看更多compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (3.5 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.