培养基MEMα，含核苷、脱氧核苷，无HEPES，1L

产品编号：12571048 品牌：invitrogen 原厂货号：12571048
产品分类：细胞,细胞培养与转染试剂 > 基础培养基 > Minimum Essential Media (MEM)
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞培养基 > MEM

包装：

1000ML

运保温度:

2-8° C

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描述：

MEM培养基（minimum essential medium）是动物细胞培养中的常用的培养基以及筛选转染的DHFR阴性细胞。MEM α被用于多种悬浮和贴壁的哺乳动物细胞培养，包括角化细胞,原代大鼠星形胶质细胞和人类黑色素瘤细胞。

MEM α 改良配方如下：

With

• Ribonucleosides

• Deoxyribonucleosides

• Phenol Red

• L-glutamine

完整配方可查看:12571 - MEM alpha, nucleosides

Gibco® MEM α是MEM的改良版。包含非必需氨基酸、丙酮酸钠、硫辛酸、维生素B12 、生物素、维生素C。无核苷MEM α可用于筛选 DG44和DHFR阴性细胞。培养基中有Earle’s salts。

MEM α不含蛋白质、脂类、生长因子等。因此使用时需添加补充添加剂，一般为10%FBS。DMEM使用碳酸氢钠缓冲体系(2.2 g / L)，因此在 5-10% CO₂ 环境下培养细胞。

原厂资料：

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. Life Technologies offers a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With

• Ribonucleosides

• Deoxyribonucleosides

• Phenol Red

• L-glutamine

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® MEM α product made in our Scotland facility (22571-038). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.