Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. Life Technologies offers a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
With Without
• Phenol Red • Ribonucleosides
• L-glutamine • Deoxyribonucleosides
The complete formulation is available.
Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® MEM α product made in our Scotland facility (22561-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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